Cytoprotective effect of 20S-Rg3 on benzo[a]pyrene-induced DNA damage.

Production of oxidative DNA damage during the metabolic activation of benzo[a]pyrene in human mammary epithelial cells correlates with cell killing.

We have studied the generation of reactive oxygen species during the metabolism of a carcinogen, benzo[a]pyrene, by human mammary epithelial cells. We have quantitated the production of one type of oxidative DNA damage, thymine glycols, by using a monoclonal antibody specific to this base modification. Thymine glycols were produced in DNA in a dose-dependent manner after exposure of human mamma...

ELECTROCHEMICAL DETECTION OF BENZO[A]PYRENE-INDUCED DNA DAMAGE AT TP53 OLIGOMERS: IMPACT OF 5' – METHYL CYTOSINE AND BIOACTIVATION ON THE GENOTOXICITY PROCESS. By

Epigallocatechin-3-gallate reduces DNA damage induced by benzo[a]pyrene diol epoxide and cigarette smoke condensate in human mucosa tissue cultures.

Although epidemiological studies indicate cancer preventive effects of diets rich in fruit and vegetables, large clinical intervention studies conducted to evaluate dietary supplementation with micronutrients, mostly vitamins, showed disappointing results in large parts. In contrast, there is encouraging epidemiologic data indicating great chemopreventive potential of a large group of phytochem...

Biochemical Activation of Aryl Hydrocarbon Hydroxylase Activity, Cellular Distribution of Polynuclear Hydrocarbon Metabolites, and DMA Damage by Polynuclear Hydrocarbon Products in Human Cells in V/fro1

Carcinogenic polynuclear hydrocarbons [7,12-dimethylbenzanthracene, 3-methylcholanthrene, and benzo(a)pyrene] were added to human skin fibroblast cell cultures. Only benzo(a)pyrene at 10 ¿ig/mlor above induced mixed-function hydroxylase activity, altered cell prolifer ation kinetics, and caused DNA damage as measured by altered grain count and bromodeoxyuridine incorporation. 3-Methylcholanthr...

Age-related negative associations between parameters of cytogenetic damage and ex vivo (+/-)-anti-benzo(a)pyrene diolepoxide-induced unscheduled DNA synthesis in smoking humans.

Chemical or physical modification of DNA may cause an increase in genomic mutations or other genetic alterations, which may ultimately result in the onset of cancer. To avoid these deleterious effects of DNA damage, humans possess DNA repair mechanisms. Decreased DNA repair, induced ex vivo by UV light or ionizing radiation in human peripheral blood lymphocytes (PBLs), has been associated with ...